Post Office Limited announced yesterday that it was going ahead with plans to shut the Main Road outlet permanently as part of a wide-ranging review to streamline their national operation.
Several post offices have already been closed in Northampton, but the announcement that the Duston branch was planned to go provoked by far the biggest public outcry.
A public consultation was launched by Post Office Limited, and talks were held with members of the Public Sector Alliance and Northampton South Labour MP Tony Clarke, who were campaigning to keep the branch open.
Jill Goodyer, who runs Goodyer Travel Agents in Main Road, was involved in the campaign to save the post office, and helped collect a petition with almost 1,800 signatures.
The 54-year-old, who grew up in the village and now lives in Holmleigh Close, Duston, said: "We all thought it was a foregone conclusion, but we could not let it go without some sort of protest in the village.
"It seems as though they (Post Office Limited) were just going through the motions.
"I grew up in the village and I know how things used to be, there was a great community spirit. It is such a shame that the post office is going, and it will be awful for the community."
Martin Nash, who owns the Duston Gallery in Main Road, has used the post office daily to send his artwork across the globe as far afield as America and across Europe.
Mr Nash's parents, 77-year-old Joan and Bill Nash, aged 83, collect their pensions from the branch.
The 41-year-old artist, who lives in Scaldwell, said: "The post office has been vital for the business. Other shops such as the hardware store have closed down, and there have been fewer and fewer people coming to the village.
"I have had to rely on the internet more and more to sell my work.
"But it is the elderly people who I feel sorry for. I can get to the other post offices which are fairly close by, but that will not be the case for a lot of pensioners.
"I do feel that Post Office Limited were paying lip service to us, and that the branch was always going to close."
The post office in Duston will close for the last time on Tuesday, September 9. The sub-post master Allan Walden yesterday declined to comment.